3 Dimensional Microscopy, Serial Block Face SEM
نویسندگان
چکیده
منابع مشابه
Three dimensional electron microscopy of cellular organelles by serial block face SEM and ET
Novel serial imaging methods using scanning electron microscopy may be used for 3D morphological studies of plastic embedded biological specimens. Serial block face SEM has already proven an undisputable position in 3D-EM studies of tissues, but it has a lot of potential in cell biology applications too. Electron tomography can be used to complement those studies where higher resolution is need...
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Electron microscopy is an important modality for the analysis of neuronal structures in neurobiology. We address the problem of tracking axons across large distances in volumes acquired by serial block-face scanning electron microscopy (SBFSEM). Tracking, for this application, is defined as the segmentation of an axon that spans a volume using similar features between slices. This is a challeng...
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Three-dimensional (3D) structural information on many length scales is of central importance in biological research. Excellent methods exist to obtain structures of molecules at atomic, organelles at electron microscopic, and tissue at light-microscopic resolution. A gap exists, however, when 3D tissue structure needs to be reconstructed over hundreds of micrometers with a resolution sufficient...
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The high-order structure of human chromosomes is an important biological question that is still under investigation. Studies have been done on imaging human mitotic chromosomes using mostly 2-D microscopy methods. To image micron-sized human chromosomes in 3-D, we developed a procedure for preparing samples for serial block-face scanning electron microscopy (SBFSEM). Polyamine chromosomes are f...
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The syncytiotrophoblast forms a continuous barrier between the maternal and fetal circulations. Here we present a serial block-face scanning electron microscopy (SBFSEM) study, based on a single image stack, showing pooling of fetal blood underneath a region of stretched syncytiotrophoblast that has become detached from the basement membrane. Erythrocytes are protruding from discrete holes in t...
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ژورنال
عنوان ژورنال: Microscopy and Microanalysis
سال: 2010
ISSN: 1431-9276,1435-8115
DOI: 10.1017/s1431927610061271